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<form id="form1" runat="server">
     <asp:GridView ID="GridView1" runat="server">   
     </asp:GridView>
</form>

要記得加 using System.Data.OleDb;
protected void Page_Load(object sender, EventArgs e)
{
    string strConn = "Provider=Microsoft.Jet.OLEDB.4.0; Data Source=C:\\Book1.xls; Extended Properties=Excel 8.0;";

    OleDbDataAdapter myCommand = new OleDbDataAdapter("SELECT * FROM [Sheet1$]", strConn);
    DataSet myDataSet = new DataSet();
    myCommand.Fill(myDataSet, "ExcelInfo");
    this.GridView1.DataSource = myDataSet.Tables["ExcelInfo"].DefaultView;
    this.GridView1.DataBind();
}